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grown under continuous mechanical impedance岡本 崇*; 鶴見 誠二*; 柴崎 杏平*; 大野 豊; Rahman, A.*
no journal, ,
機械的な刺激を与えるために透析膜でカバーした培地上で育成したシロイヌナズナの根は、エチレン反応に特徴的な形態を示した。この形態について詳しく調べたところ、エチレンの産生ではなくエチレンの情報伝達が、このエチレン応答の増強に重要な役割を果たしていることがわかった。さらに、エチレン反応の増強は、根におけるオーキシン応答にも影響を与えていることを明らかにした。以上の結果は、機械的刺激による根の生長変化におけるエチレンの役割について新たな知見を与えるものである。
L.)中村 進一*; 鈴井 伸郎; 長坂 俊紀*; 石岡 典子; 河地 有木; 松橋 信平; 服部 浩之*; 茅野 充男*; 藤巻 秀
no journal, ,
We investigated effects of GSH to Cd transport and accumulation when GSH was administered to specific organs. Plants were grown hydroponically in a green house and were treated with Cd and GSH. After 2-day treatment, we visualized Cd movement in plants using the position-emitting tracer imaging system (PETIS). Reduction of Cd transport and accumulation by GSH, administered to roots, was also confirmed by serial images of Cd movement, obtained by PETIS. Our results demonstrated that effects of GSH to Cd transport and accumulation in the plants were different in the organs where GSH was administered.
鈴井 伸郎; 河地 有木; 石井 里美; 中村 進一*; 石岡 典子; 藤巻 秀
no journal, ,
Higher plants regulate photoassimilate flow from source to sink organ in order to respond to environmental or developmental changes. An understanding of the source-sink interrelationship requires an experimental system which can measure the change of phothoassimilate flow corresponding to various conditions in source and sink organ. In this study, we adapted 
C-tracer and the positron emitting tracer imaging system (PETIS), which can obtain carbon dynamics in intact plants non-invasively and quantitatively. To manipulate the conditions in source and sink organ, we treated leaves or roots of plants (rice and soybean) with p-chlorobenzenesulfonic acid (PCMBS), an inhibitor of sucrose transporters. CO
was supplied to leaves after PCMBS treatments and the translocations of C-photoassimilates were monitored using PETIS repetitively with the same plants. We have developed an analytical method to estimate the velocity of C-photoassimilate flow from PETIS data. As a result, a gradual change in the velocity after PCMBS treatments was successfully detected. The effect of source/sink strength on the velocity of photoassimilate flow was discussed.
retrieving mechanisms at the shoot base to maintain low Naconcentration in the shoot丸山 哲平*; 鈴井 伸郎; 河地 有木; 藤巻 秀; 吉羽 雅昭*; 三輪 睿太郎*; 樋口 恭子*
no journal, ,
The Na concentration in common reed roots is similar to that in rice roots, while common reed shoots have lower Na concentration than rice shoots. It has been suggested that in common reed, Na translocation to the shoot is inhibited at the shoot base (SB). To evaluate the SB contribution to the inhibition of Na translocation to the shoots and determine the Na translocation pathway in the SB, we analyzed the kinetics of 
Na in common reed and in rice under the 50mM NaCl stress condition by performing a non-invasive tracer experiment with the positron-emitting tracer imaging system (PETIS). The PETIS experiments showed that Na accumulation is slower in the SB and the shoots of common reed than in those of rice. The Na accumulated in the shoots of both species was not actively excluded under the continuous mild saline condition. Modeling of the Na fluxes within the SB revealed that common reed strongly retrieved Na from the xylem at the SB.